OTS refers to a technique refined by queen-rearing expert Mel Disselkoen called On-The-Spot queen rearing. This technique is used with a hive-splitting system to encourage queenless colonies to produce a new, robust queen. Mel developed the OTS system after studying other methods of queen rearing used by G.M. Doolittle and Dr. C.C. Miller. Benefits of using the OTS system include expanding your apiary, increasing honey yield, and producing post-solstice queens (queens eclosed after the summer solstice/end of June in MI) that typically have better winter survival rates.
The crux of the OTS method is using a notching method to elongate cells with either eggs or less than 3-day-old larvae to make room for the bees to turn these cells into queen cells. To do this, you would press the sharp edge of the flat, prying end of your hive tool straight down into the wax bottom wall of the cells and then tilt your hive tool down and back to flatten the area underneath the cells. This artificially elongates the cell and again gives space for the bees to create a queen cell.
Here are some tips to remember when using the OTS method. Never allow a split or a start to make a new queen, but encourage full-sized hives with a minimum of 4 frames of brood to make new queens. OTS may encourage bees to produce many queen cells, but you want to leave only 1-2 of the largest cells to discourage fighting amongst the virgin queens. Notching will only encourage bees to make queen cells if there is no queen present in the hive. Bees need an abundance of food and nurse bees to rear a robust queen.
More Information: https://www.mdasplitter.com/
Any fertilized honey bee egg can become a queen bee. All larvae are fed royal jelly (a protein-rich substance secreted from a gland in the nurse bee’s head) for the first three days after the egg hatches. After 3 days, larvae chosen to become queens continue to be fed royal jelly while most female larvae start to be fed bee bread (a fermented mixture of pollen and honey) and will become worker bees.
The queen bee does not “run” the hive, but she does somewhat control the actions of the workers through the pheromones she excretes. Queens emit many pheromones that let the workers know she is present and healthy. When the levels of queen pheromones she gives off goes down, the workers will supercede her (i.e. make a new queen). The workers will also make a new queen if the queen dies suddenly or when they are going to swarm. Typically, a hive will make many new queens to ensure the successful raising of a queen, and the first queen to eclose will find the other new queens (sometimes still in their cells) and sting them to death.
There is usually only one queen in a hive. She is the largest bee because she has an abdomen full of eggs and sperm and is the only female able to lay fertilized eggs. A healthy queen can lay 3,000 eggs per day. About a week after emerging (eclosing), a virgin queen will leave the hive on one or more mating flights. On these flights she will mate with between 10 and 20 drones from other colonies. If the colony doesn't swarm, this is the only time the queen will ever leave the hive.
More information: https://extension.psu.edu/an-introduction-to-queen-honey-bee-development